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Clonaggio molecolare · Joining into Selection screen. "V Restriction endonuclease digestion Horn o...

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Clonaggio molecolare

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Page 1: Clonaggio molecolare · Joining into Selection screen. "V Restriction endonuclease digestion Horn o polym er tailing with recombinant phage DNA Hybridization Mechanical

Clonaggio molecolare

Page 2: Clonaggio molecolare · Joining into Selection screen. "V Restriction endonuclease digestion Horn o polym er tailing with recombinant phage DNA Hybridization Mechanical

DNA genomico

E.coli 4.0 X 103

S.cerevisiae 1.3 X 104

A.thaliana 1.3 X 105

Z.mais 1.5 X 107

D.melanogaster 1.8 X 105

Topo 2.3 X 106

Uomo 2.8 X 106

X.lavis 3.0 X 106

Dimensioni del DNA genomico

Page 3: Clonaggio molecolare · Joining into Selection screen. "V Restriction endonuclease digestion Horn o polym er tailing with recombinant phage DNA Hybridization Mechanical

DNA genomico

Page 4: Clonaggio molecolare · Joining into Selection screen. "V Restriction endonuclease digestion Horn o polym er tailing with recombinant phage DNA Hybridization Mechanical

DNA genomico

Sau3A: GATCBamHI:GGATCC

Page 5: Clonaggio molecolare · Joining into Selection screen. "V Restriction endonuclease digestion Horn o polym er tailing with recombinant phage DNA Hybridization Mechanical

DNA genomico

Page 6: Clonaggio molecolare · Joining into Selection screen. "V Restriction endonuclease digestion Horn o polym er tailing with recombinant phage DNA Hybridization Mechanical

Clonaggio cDNA

Page 7: Clonaggio molecolare · Joining into Selection screen. "V Restriction endonuclease digestion Horn o polym er tailing with recombinant phage DNA Hybridization Mechanical

Clonaggio cDNA

Page 8: Clonaggio molecolare · Joining into Selection screen. "V Restriction endonuclease digestion Horn o polym er tailing with recombinant phage DNA Hybridization Mechanical

cDNA: clonaggio direzionale

Page 9: Clonaggio molecolare · Joining into Selection screen. "V Restriction endonuclease digestion Horn o polym er tailing with recombinant phage DNA Hybridization Mechanical

cDNA cloning: 5’

Page 10: Clonaggio molecolare · Joining into Selection screen. "V Restriction endonuclease digestion Horn o polym er tailing with recombinant phage DNA Hybridization Mechanical

cDNA cloning: RACE

Page 11: Clonaggio molecolare · Joining into Selection screen. "V Restriction endonuclease digestion Horn o polym er tailing with recombinant phage DNA Hybridization Mechanical

Screening: ibridazione di colonie

Page 12: Clonaggio molecolare · Joining into Selection screen. "V Restriction endonuclease digestion Horn o polym er tailing with recombinant phage DNA Hybridization Mechanical

Screening: ibridazione di placche

Page 13: Clonaggio molecolare · Joining into Selection screen. "V Restriction endonuclease digestion Horn o polym er tailing with recombinant phage DNA Hybridization Mechanical

Screening: genoteche di espressione

Page 14: Clonaggio molecolare · Joining into Selection screen. "V Restriction endonuclease digestion Horn o polym er tailing with recombinant phage DNA Hybridization Mechanical

Screening: complementazione funzionale

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